how long do I have to use spores in a syringe? - Mushroom Cultivation (2024)

FAQs

How long do I have to use spores in a syringe? - Mushroom Cultivation? ›

Put enough compost in a tray to cover to a depth of about 3 inches then spray over the spore syringe to inoculate. Mix again and cover with a damp newspaper. In 2-3 weeks the mycelium should be showing signs of growth.

Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions. Ideal conditions for this phase are in a dark place and temperature between 72 F – 80 F.

Key Takeaways. Mushroom spore syringes can last 4 to 12 months in the fridge, and high – quality ones may last up to 24 months. Keep them away from light for best results.

Colonization from a spore syringe will take anywhere from 2-6 weeks depending on how much grain is in your container, how much spore solution you inoculate with, and how optimized you are able to make the environmental conditions. Colonization from a Liquid Culture syringe will take anywhere from 2-4 weeks.

After a week to ten days, mycelium grows from the tissue and colonizes the agar. Great care should be taken to select a fruiting body of the highest quality, size, color, shape or any highly desired characteristic.

Slide those precious spore syringes into a clean ziplock bag and gently tuck them onto a fridge shelf. They'll stay happy there at an ideal temperature between 32°F to 46°F. Now let's talk dry storage – think cool cupboards or drawers away from any heat or light source.

Typically, the mycelium covers the baglog about 40 days after inoculation-some media, even less than 40 days.

If the spore germinates, then it is considered to have been dormant whereas if it does not, then it is considered to have been dead.

A clean and uncontaminated spore syringe should generally maintain transparency or slight coloration without any visible signs of contamination or irregularities.

Some people suggest using an entire cc of solution per jar, however we have had great success with only . 5cc each. Be careful that nothing but the jar and substrate touch the needle, and re-cap it immediately after using it to avoid contaminating the needle. Also be careful of using too much spore solution.

Can you inject spores into substrate? ›

Simply inject your favorite spores or live culture into the bag, wait for the mycelium to colonize, slice a hole in the bag just above the substrate for fresh air exchange, and wait for your mushrooms to fruit!

While the above process is a tried and tested way to create liquid cultures, those looking to forgo this method can find a much simpler way using pre-made spore syringes and liquid culture vials. All you need to do is inject around 1ml of spores into the liquid culture vial, then shake.

Spore syringes are typically sold in 10cc syringes. For example, 12 substrate jars would only require one spore syringe to use.

Light: While mycelium doesn't require light to grow, a small amount can help stimulate growth. 5. Nutrition: Using a nutrient-rich substrate can also enhance growth. Remember, it's important to maintain sterile conditions to prevent contamination.

Here are some key factors: 1. Temperature: The optimal temperature for mycelium growth is usually between 24-27°C. 2. Humidity: High humidity levels (around 95-100%) are ideal for mycelium growth.

Initial mycelium growth is visible within 1-3 weeks of inoculating your pod with spores or liquid culture. Full colonization usually occurs within 4-5 weeks, though it can sometimes take longer.

-Label the plate with the species of the culture and the date inoculated. -Store the plate upside down to avoid condensation on the lid. It will take up to two weeks for the mycelium to cover the petri plate.

begins, you should see white growth within 5-7 days and should quickly spread and fully colonize in anywhere from 14-21 days.